Purity and net peptide content calculator
Mass arithmetic for a weighed powder. You enter every number, including the percentages; nothing is pre-filled or suggested. The tool does not know what your material contains. Use a decimal point (2.5, not 2,5).
Gross mass and a net peptide content (and, optionally, a purity) to the mass of peptide in the powder.
Result
Enter your numbers and choose Calculate.
Formulas
- Peptide mass: gross mass × net peptide content ÷ 100.
- Main-peptide mass: peptide mass × purity ÷ 100.
- Gross mass to weigh: wanted mass ÷ (net peptide content ÷ 100 × purity ÷ 100).
- Content from components: 100 − water − counter-ion − other.
- Corrected concentration: gross mass ÷ volume × net peptide content ÷ 100.
Read this first. Purity from a chromatogram is an area percent, not a mass fraction, so multiplying it by a content is an approximation. Use values measured on the same material. If you do not have a net peptide content, leave the calculation alone rather than guessing one. See purity versus net peptide content.
Research use only. For laboratory research use only. Not for human or veterinary use. This page performs arithmetic on the numbers you enter and gives no recommendations about amounts. Check results against your own protocol and records. All laboratory tools · Laboratory guides · Glossary