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ARA-290 reconstitution calculator

Solution arithmetic for lyophilized ARA-290: concentration from vial mass and solvent volume, the solvent volume for a target concentration, and aliquot volumes. The molecular weight (~1257 Da) is filled in so results also show millimolar values. You enter every amount; nothing is suggested.

Choose a calculation

Sample mass and solvent volume to concentration.

Result

Enter your numbers and choose Calculate.

Formulas

  • Concentration: mg/mL = mass (mg) ÷ volume (mL). µg/mL = mg/mL × 1000.
  • Solvent volume: volume (mL) = mass (mg) ÷ target concentration (mg/mL).
  • Aliquot volume: volume (mL) = mass wanted (mg) ÷ solution concentration (mg/mL). µL = mL × 1000.
  • Molar: mM = mg/mL ÷ molecular weight (g/mol) × 1000.
  • Units: 1 mg = 1000 µg. 1 mL = 1000 µL.

Worked arithmetic for ARA-290

Each cell is simply mass ÷ volume, with the molar value from the listed molecular weight (~1257 Da). Rows use round example masses. The table illustrates the formula; it is not a recommendation for any amount or volume.

ARA-290: concentration for each vial mass and solvent volume
Vial mass1 mL solvent2 mL solvent3 mL solvent5 mL solvent
5 mg5 mg/mL
5,000 µg/mL · 3.98 mM
2.5 mg/mL
2,500 µg/mL · 1.99 mM
1.67 mg/mL
1,670 µg/mL · 1.33 mM
1 mg/mL
1,000 µg/mL · 0.796 mM
10 mg10 mg/mL
10,000 µg/mL · 7.96 mM
5 mg/mL
5,000 µg/mL · 3.98 mM
3.33 mg/mL
3,330 µg/mL · 2.65 mM
2 mg/mL
2,000 µg/mL · 1.59 mM

Formulas used

  • Concentration: mg/mL = vial mass (mg) ÷ solvent volume (mL).
  • Molar: mM = mg/mL ÷ 1,257 g/mol × 1000.
  • Aliquot volume: µL = mass wanted (µg) ÷ concentration (µg/mL) × 1000.

Stated vial mass is gross lyophilized powder. Net peptide content can be lower because of counter-ions and water; the purity and net peptide content calculator corrects a nominal concentration if you have those values.

ARA-290 at a glance

Molecular weight~1257 Da
Molecular formulaC51H84N16O21
Catalogue statusComing soon: vial size and pricing not yet listed

ARA-290 single vial (coming soon) · ARA-290 research overview · Reconstitution guide · Reconstitution math explained · Shipping & storage on arrival · Glossary

ARA-290 calculator FAQ

How is ARA-290 concentration calculated after reconstitution?

Divide the vial mass by the solvent volume. For example, 5 mg in 2 mL gives 2.5 mg/mL (2,500 µg/mL). This is unit arithmetic only, not a recommendation for any amount.

How do I convert ARA-290 mg/mL to millimolar?

Divide mg/mL by the molecular weight (~1257 Da) and multiply by 1000. 2.5 mg/mL ÷ 1,257 g/mol × 1000 = 1.99 mM.

Why can the real concentration be lower than the calculated value?

The stated vial mass is gross lyophilized powder. Counter-ions (such as acetate or TFA) and residual water mean net peptide content is lower than gross mass, so the true peptide concentration can be lower. The purity and net peptide content calculator applies that correction if you have the values.

Is this calculator for human or veterinary use?

No. ARA-290 is sold for laboratory research use only. This page performs solution arithmetic on the numbers you enter and gives no dosing or usage guidance.

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Background reading

The arithmetic on this page is explained, with its assumptions, in these guides.

Research use only. For laboratory research use only. Not for human or veterinary use. This page performs unit arithmetic on the numbers you enter and gives no recommendations about amounts or dosing. Check results against your own protocol and records.